CHROMATOGRAPHIC AND ELECTROPHORETIC TECHNIQUES

Resolution of isomers of sorbitolparaben esters by chromatographic and electrophoretic techniques.

Pharmaceutical preparations usually contain preservatives and sweeteners. When parabens are used as preservatives and a polyol as a sweetener, a transesterification reaction may happen, yielding the transester polyol-paraben. The products formed in the transesterification reaction of methylparaben and sorbitol were analyzed by micellar electrokinetic chromatography and by HPLC. Up to six positi...

kallikrein-like proteinases: purification and chromatographic and electrophoretic methods

Kall ikreins and kall ikrein-l ike e n z y m e s make up a fami ly o f serine prote inases present in t i s sues and body fluids o f m a m m a l s and in s o m e snake venoms . This review deals with the procedures o f purification, detect ion and determinat ion o f these e n z y m e s by chromatographic and electrophoretic methods . The procedures are reported in tables, descr ibed and d i scu...

Chromatographic and electrophoretic separation of creatine kinase isoenzymes compared.

We compared two techniques for separating and evaluating serum creatine kinase isoenzymes--fluorometric agarose electrophoresis and Sephadex chromatography--in 50 patients, 25 of whom had confirmed acute myocardial infarction. In every case isoenzyme MB (heart isoenzyme) was detected with equal sensitivity by either procedure. Evidently, only the presence or absence of MB is clinically signific...

Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia.

Studies of the hemoglobin pattern in Brazilian reptiles are important for determining ecological and phylogenetic relationships, but they are scarce. Peripheral blood samples were obtained from 7 males and 18 females of Rhinoclemmys punctularia. The hematological profile was based on the total hemoglobin and hematocrit values. The hemoglobin profile was obtained using electrophoretic procedures...

Electrophoretic Techniques in Microbial Ecology